Tech Spotlight: Affinity Tag-Based Purification Method for Native RNA Molecules

Production of pure native single species of RNA is necessary for a myriad of biochemistry, molecular biology, and structural biology experiments, but is time- and labor-intensive and the current denaturing method of purification is not high-throughput. Drs. Rob Batey and Jeff Kieft of the University of Colorado have developed a general method for purifying small or large quantities of any RNA oligonucleotide under nondenaturing conditions using a unique RNA affinity tag. This protocol may revolutionize RNA purification in much the same way removable affinity tags allow the rapid purification of proteins via affinity chromatography.

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