A research group led by Dr. Ryan Gill of the University of Colorado has utilized genome-wide tools and analysis techniques to engineer bacterial strains with increased tolerance to acetate, furfural, and ethanol. Acetate and furfural tolerance are important for efficient conversion of pretreated cellulosic biomass, and ethanol tolerance is important for the production of ethanol as a biofuel. This technology offers bacterial strains capable of tolerating industrially-relevant concentrations of acetate, furfural, and ethanol, along with other inhibitors found in cellulosic hydrolysate.
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